Mycobacterial cell division arrest and smooth-to-rough envelope transition using CRISPRi-mediated genetic repression systems Point, V., Achache, W., Laudouze, J., Sepulveda Ramos, E., Maziero, M., Crauste, C., Canaan, S. and Santucci, P. The genetic basis underlying nontuberculous mycobacteria (NTM) pathogenesis remains poorly understood. This gap in knowledge has been partially filled over the years through the generation of novel and efficient genetic tools, including the r

Fluorescence-based CRISPR interference system for controlled genetic repression and live single-cell imaging in mycobacteria Laudouze, J.; Point, V.; Achache, W.; Crauste, C.; Canaan, S.; Santucci, P. In this research letter, we report the development and validation of a new subset of fluorescence-based CRISPR interference (CRISPRi) tools for our scientific community. The pJL series is directly derived from the original pIRL CRISPRi vectors and conserves all the elements to p