Green Synthesis, Antibacterial Activity, and Molecular Docking Study of γ-Phosphonyloxime Derivatives Wahbi, A.; Mhamdi, A.; Tahri, W.; Dridi, I.; Dridi, M. A.; Kamisky, W.; Kaabi, K.; Chatti, A.; Nasr, C. B.; Cavalier, J.-F.; Touil, S. Herein, we report an efficient, fast, and green approach to synthesize γ-phosphonyloximes (2) via microwave-assisted oximation of γ-phosphonylketones under solvent-free conditions. This method achieved high yields (up to 95%) in just 5 min. Su

Fast-growing intracellular Mycobacterium tuberculosis populations evade antibiotic treatment Day, N. J.; Pradel, B.; Luk, C. H.; Santucci, P.; Fearns, A.; Rodgers, A.; Aylan, B.; Botella, L.; Vaubourgeix, J.; Ortiz Canseco, J.; Athanasiadi, N.; Gutierrez, M. G. Slow growth rates are considered a hallmark of Mycobacterium tuberculosis (Mtb) and have been historically associated with persistence and subsequent drug tolerance. Despite the fundamental role intracellular growth pl

A simple multi-cloning strategy for gene expression and subcellular localization in mycobacteria Deschutter, M.S.; Beaumale, E.; Canaan, S.; Gago, G.; Santucci, P. Genetic manipulation of mycobacteria remains critical in functional genomics and modern microbiology. However, this process is often laborious due to limited cloning flexibility and low throughput. Here, we describe a streamlined cloning platform for expressing a gene of interest either alone or fused to a monomeri

Oxadiazolone Derivatives: Powerful Tools to Reduce Staphylococcus aureus Infection Forest E., Lehoux J., Faion L., Ranava D., Mohymont V., Durand T., Audebert S., Camoin L., Crauste C., Canaan S., Brunel J. M., Van Bambeke F., Bolla J.-M., Cavalier, J.-F. Staphylococcus aureus is a Gram-positive opportunistic pathogen and a top priority bacterium in the fight against antimicrobial resistance. Its high propensity to develop resistance, its high virulence, and its ability to fo

Elaboration of antibacterial polyurethanes for medical devices by a scalable process Caron B., Maresca M., Leroux A., Lemesle M., Coussegal J. L., Fontaine L., Murphy E. A., Bates C. M., Nguyen P. H., Kohl P. A., Soppera O., Canaan S., Poncin I., Guillaneuf Y. Lefay C. Medical devices are critical for patient survival in hospitals, yet they represent a leading cause of infections. Polyurethanes (PU), owing to their alternating hard- and soft-segment architecture, enable the f

Identification of Fluoro-methoxy-phenyl-substituted N-sulfonylpiperidine-mono-spiro-1,2,4,5-tetraoxanes as Multispecies-targeting Molecules Tiwari M. K., Bakun P., Forest E., Bola J.-M., Murias M., Kucinska M., Zgoła-Grześkowiak A., Cicha E., Marczak Ł., Cavalier J.-F., Grellier P., Goslinski T. Herein, we report a novel series of heterocyclic fluoro-methoxy-phenyl-substituted N-sulfonylpiperidine monospiro-1,2,4,5-tetraoxanes (5a-l; 50-93%) that have been successfully synthe

Mycobacterial cell division arrest and smooth-to-rough envelope transition using CRISPRi-mediated genetic repression systems Point, V., Achache, W., Laudouze, J., Sepulveda Ramos, E., Maziero, M., Crauste, C., Canaan, S. and Santucci, P. The genetic basis underlying nontuberculous mycobacteria (NTM) pathogenesis remains poorly understood. This gap in knowledge has been partially filled over the years through the generation of novel and efficient genetic tools, including the r

Bioactive Compounds Discovery from French Guiana Plant Extracts Through Antitubercular Screening and Molecular Networking Breaud, C.; Saunier, C.; Baghdikian, B.; Mabrouki, F.; Bertolotti, M.; Royer, M.; Silland, P.; Maresca, M.; Garaev, E.; Cavalier, J.-F.; Canaan, S.; Bun-Llopet, S.-S.; Garayev, E. Tuberculosis (TB) is still a significant public health threat, with rising drug resistance and high incidence in multiple areas worldwide. In the search for novel antitubercular

The polyamino-isoprenyl enhancer NV716 enables the antibacterial activity of two families of multi-target inhibitors against the ESKAPEE...

Mapping of Mycobacterial Enzymes Involved in Triacylglycerol Accumulation as Intrabacterial Lipid Inclusions Using Activity-Based...