A dTDP-L-rhamnose 4-epimerase required for glycopeptidolipid biosynthesis in Mycobacterium abscessus Aguilera-Correa, J.J.; Wei, F.; Leclercq, L.D.; Tasrini, Y.; Mullapudi, E.; Daher, W.; Nakajima, K.; Canaan, S.; Herrmann, J.L.; Wilmanns, M.; Guérardel, Y.; Wen, L.; Kremer, L. Mycobacterium abscessus  causes severe lung infections in cystic fibrosis patients and exhibits smooth (S) or rough (R) morphotypes. Disruption of glycopeptidolipid (GPL) production results in the S-to

Fluorescence-based CRISPR interference system for controlled genetic repression and live single-cell imaging in mycobacteria Laudouze, J.; Point, V.; Achache, W.; Crauste, C.; Canaan, S.; Santucci, P. In this research letter, we report the development and validation of a new subset of fluorescence-based CRISPR interference (CRISPRi) tools for our scientific community. The pJL series is directly derived from the original pIRL CRISPRi vectors and conserves all the elements to p