Fast-growing intracellular Mycobacterium tuberculosis populations evade antibiotic treatment Day, N. J.; Pradel, B.; Luk, C. H.; Santucci, P.; Fearns, A.; Rodgers, A.; Aylan, B.; Botella, L.; Vaubourgeix, J.; Ortiz Canseco, J.; Athanasiadi, N.; Gutierrez, M. G. Slow growth rates are considered a hallmark of Mycobacterium tuberculosis (Mtb) and have been historically associated with persistence and subsequent drug tolerance. Despite the fundamental role intracellular growth pl

A simple multi-cloning strategy for gene expression and subcellular localization in mycobacteria Deschutter, M.S.; Beaumale, E.; Canaan, S.; Gago, G.; Santucci, P. Genetic manipulation of mycobacteria remains critical in functional genomics and modern microbiology. However, this process is often laborious due to limited cloning flexibility and low throughput. Here, we describe a streamlined cloning platform for expressing a gene of interest either alone or fused to a monomeri

Bioactive Compounds Discovery from French Guiana Plant Extracts Through Antitubercular Screening and Molecular Networking Breaud, C.; Saunier, C.; Baghdikian, B.; Mabrouki, F.; Bertolotti, M.; Royer, M.; Silland, P.; Maresca, M.; Garaev, E.; Cavalier, J.-F.; Canaan, S.; Bun-Llopet, S.-S.; Garayev, E. Tuberculosis (TB) is still a significant public health threat, with rising drug resistance and high incidence in multiple areas worldwide. In the search for novel antitubercular

Unraveling Mycobacterium tuberculosis acid resistance and pH homeostasis mechanisms Laudouze, J.; Canaan, S.; Gouzy, A.; Santucci, P. ...

Potency of all-D amino acid antimicrobial peptides derived from the bovine rumen microbiome on tuberculous and non-tuberculous...

Antitubercular potential and pH-driven mode of action of salicylic acid derivatives Laudouze, J.; Francis, T.; Forest, E.; Mies, F.; Bolla, J.M.; Crauste, C.; Canaan, S.; Shlyonsky, V.; Santucci, P.; Cavalier, J.-F. In the search for new antituberculosis drugs with novel mechanisms of action, we evaluated the antimycobacterial activity of a panel of eight phenolic acids against four pathogenic mycobacterial model species, including Mycobacterium tuberculosis . We demonstrated

Intrabacterial lipid inclusion-associated proteins: A core machinery conserved from saprophyte Actinobacteria to the human pathogen...

High content quantitative imaging of Mycobacterium tuberculosis responses to acidic microenvironments within human macrophages Aylan, B.; Botella, L.; Gutierrez, M. G.; Santucci, P. Intracellular pathogens such as Mycobacterium tuberculosis (Mtb) have evolved diverse strategies to counteract macrophage defence mechanisms including phagolysosomal biogenesis. Within macrophages, Mtb initially resides inside membrane-bound phagosomes that interact with lysosomes and become

Lysosomal damage drives mitochondrial proteome remodelling and reprograms macrophage immunometabolism Bussi, C.; Heunis, T.; Pellegrino,...

Direct capture, inhibition and crystal structure of HsaD (Rv3569c) from M. tuberculosis Barelier, S.; Avellan, R.; Gnawali, G. R.;...