Fluorescence-based CRISPR interference system for controlled genetic repression and live single-cell imaging in mycobacteria Laudouze, J.; Point, V.; Achache, W.; Crauste, C.; Canaan, S.; Santucci, P. In this research letter, we report the development and validation of a new subset of fluorescence-based CRISPR interference (CRISPRi) tools for our scientific community. The pJL series is directly derived from the original pIRL CRISPRi vectors and conserves all the elements to p