Fast-growing intracellular Mycobacterium tuberculosis populations evade antibiotic treatment Day, N. J.; Pradel, B.; Luk, C. H.; Santucci, P.; Fearns, A.; Rodgers, A.; Aylan, B.; Botella, L.; Vaubourgeix, J.; Ortiz Canseco, J.; Athanasiadi, N.; Gutierrez, M. G. Slow growth rates are considered a hallmark of Mycobacterium tuberculosis (Mtb) and have been historically associated with persistence and subsequent drug tolerance. Despite the fundamental role intracellular growth pl

A simple multi-cloning strategy for gene expression and subcellular localization in mycobacteria Deschutter, M.S.; Beaumale, E.; Canaan, S.; Gago, G.; Santucci, P. Genetic manipulation of mycobacteria remains critical in functional genomics and modern microbiology. However, this process is often laborious due to limited cloning flexibility and low throughput. Here, we describe a streamlined cloning platform for expressing a gene of interest either alone or fused to a monomeri

Oxadiazolone Derivatives: Powerful Tools to Reduce Staphylococcus aureus Infection Forest E., Lehoux J., Faion L., Ranava D., Mohymont V., Durand T., Audebert S., Camoin L., Crauste C., Canaan S., Brunel J. M., Van Bambeke F., Bolla J.-M., Cavalier, J.-F. Staphylococcus aureus is a Gram-positive opportunistic pathogen and a top priority bacterium in the fight against antimicrobial resistance. Its high propensity to develop resistance, its high virulence, and its ability to fo

High content quantitative imaging of Mycobacterium tuberculosis responses to acidic microenvironments within human macrophages Aylan, B.; Botella, L.; Gutierrez, M. G.; Santucci, P. Intracellular pathogens such as Mycobacterium tuberculosis (Mtb) have evolved diverse strategies to counteract macrophage defence mechanisms including phagolysosomal biogenesis. Within macrophages, Mtb initially resides inside membrane-bound phagosomes that interact with lysosomes and become

Synthesis and Biological Characterization of Fluorescent Cyclipostins and Cyclophostin Analogues: New Insights for the Diagnosis of...